1. Understanding carbapenem-resistant hypervirulent Klebsiella pneumoniae: Key virulence factors and evolutionary convergencehLife | 破解耐药高毒力肺炎克雷伯菌毒力因子与进化机制揭秘通信作者肖永红本文系统阐述了耐碳青霉烯类高毒力肺炎克雷伯菌CR-hvKP的关键毒力因子及其进化整合机制。CR-hvKP的毒力相关基因分布于核心基因组染色体及辅助基因组如质粒、整合性接合元件 [ICEKp]中。这些遗传元件编码荚膜、脂多糖、铁载体、1/3型菌毛及基因毒素柯杆菌素colibactin等毒力因子赋予菌株高致病潜力。 CR-hvKP的形成源于持续的进化压力以及携带碳青霉烯酶基因或高毒力基因的质粒交换。目前认为耐药与高毒力表型在同一菌株中汇合的机制主要有三种1碳青霉烯耐药质粒水平转移至高毒力肺炎克雷伯菌2高毒力质粒转移至耐碳青霉烯肺炎克雷伯菌3菌株获得同时携带耐药及毒力基因的嵌合/杂合质粒。目前耐药与高毒力谱系在单一菌株中的融合现象日益增多对公共卫生构成严重威胁。CR-hvKP的广泛流行主要归因于其高度可变的基因组这种可塑性源于点突变、同源重组以及质粒、插入序列、原噬菌体等多种可移动遗传元件的频繁转移。引用Chen T, Ying L, Xiong L, et al. Understanding carbapenem-resistant hypervirulent Klebsiella pneumoniae: Key virulence factors and evolutionary convergence. hLife 2024; 2: 611–624.2. WHO revised bacterial priority pathogens list to encourage global actions to combat AMR通信作者肖永红The World Health Organization (WHO) has updated its Bacterial Priority Pathogens List (BPPL) for 2024 to combat antimicrobial resistance (AMR), focusing on One Health criteria such as treatability, mortality, and resistance trends. The list categorizes pathogens as critical, high, or medium priority, emphasizing the need for new antimicrobials. Notable inclusions are carbapenem-resistant Acinetobacter baumannii (CRAB), carbapenem-resistant Enterobacterales (CRE), and third-generation cephalosporin-resistant Enterobacterales (3GCRE). The AMR crisis, especially with multidrug-resistant gram-negative bacteria, persists, highlighting the urgency for global action, investment, and RD in diagnostics and therapeutics.引用Ma Y, Chen P, Mo Y, et al. WHO revised bacterial priority pathogens list to encourage global actions to combat AMR. hLife 2024; 2: 607–610.3. Personalized bacteriophage therapy for chronic biliary tract Pseudomonas aeruginosa infectionshLife | 胡必杰与乐率等研究团队合作发现对抗超级细菌的利器首例超级细菌胆道感染的噬菌体治疗案例通信作者朱同玉胡必杰乐率本研究首次证实了噬菌体疗法在胆道感染中的安全性和有效性为治疗提供了新的思路。尽管完全清除感染仍具挑战但噬菌体疗法在缓解症状和减少细菌载量方面效果显著。引用Li N, Li L, He B, et al. Personalized bacteriophage therapy for chronic biliary tract Pseudomonas aeruginosa infections. hLife 2025; 3: 275–283.4. RmpA drives metabolic reprogramming to modulate the phenotypic switch between hypermucoviscosity and biofilm formation in hypervirulent Klebsiella pneumoniaehLife | 冯婕研究团队揭示RmpA是操控高毒力肺炎克雷伯菌在高粘液性与生物膜间切换的代谢开关通信作者冯婕本文通过在高毒力肺炎克雷伯菌中构建针对rmpA基因的dCas9敲低菌株结合RNA-seq和ChIP-seq技术揭示了RmpA作为“代谢中枢调控器”的双重功能。引用Yao S, Huang J, Geng J, et al. RmpA drives metabolic reprogramming to modulate the phenotypic switch between hypermucoviscosity and biofilm formation in hypervirulent Klebsiella pneumoniae. hLife 2025; 3: 504–516.5. Carbapenem-resistant Klebsiella pneumoniae ST11 index from a single strain enhances rapid parallel evolution during persistent infectionhLife | 深圳三院曲久鑫研究团队揭示高毒高耐肺炎克雷伯菌ST11持续感染过程中的宿主内进化通信作者曲久鑫本文通过全基因组测序WGS分析了从一名持续感染患者体内时序性分离的一系列肺炎克雷伯菌菌株揭示并表征了肺炎克雷伯菌基因组、耐药表型和毒力在宿主内分化和适应过程并呼吁借鉴病毒学从准种理论的角度认识病原细菌持续性感染。引用Li L, Jiang Z, Wang X, et al. Carbapenem-resistant Klebsiella pneumoniae ST11 index from a single strain enhances rapid parallel evolution during persistent infection. hLife 2025; 3: 517–520.6.Heterogeneity and clinical genomics of blaKPC-2-producing, carbapenem-resistant Pseudomonas aeruginosahLife | 揭秘产blaKPC-2的碳青霉烯耐药铜绿假单胞菌的异质性与临床基因组学之谜通信作者黄曼、冯友军本文报道了KPC-2耐药质粒在碳青霉烯类耐药铜绿假单胞菌CRPA中的分布和遗传特征为研究我国临床ICU环境中CRPA传播机制提供了重要的基因组学视角。引用Huang M, Liu L, Li X, et al. Heterogeneity and clinical genomics of blaKPC-2-producing, carbapenem-resistant Pseudomonas aeruginosa. hLife 2024; 2: 314–319.7.Convergence of carbapenem resistance and hypervirulence in Klebsiella pneumoniae: An emerging public health threathLife | 高毒高耐肺炎克雷伯菌的全球播散及应对策略通信作者王明贵高耐高毒肺炎克雷伯菌的出现和广泛传播是潜在的公共卫生危机这类菌株正悄然改变临床感染格局。未来研究和防控策略可聚焦于基于CRISPR-Cas或毒素-抗毒素系统的去定植研究、针对毒力因子的靶向治疗研究、结合新型分子检测与监测网络的早期预警系统的建立等。只有通过加强基础研究、研发诊断手段、推动多学科协作才能有效遏制其蔓延守护临床安全与公共卫生防线。引用Jiang J, Zhang J, Sun Z, et al. Convergence of carbapenem resistance and hypervirulence in Klebsiella pneumoniae: An emerging public health threat. hLife 2025; 3: 501–503.8. Impact of COVID-19-related nonpharmaceutical interventions on diarrheal diseases and zoonotic SalmonellahLife | COVID-19相关非药物干预措施对急性腹泻和人畜共患沙门菌的影响通信作者乐敏引用Huang L, Zhou H, Chen J, et al. Impact of COVID-19-related nonpharmaceutical interventions on diarrheal diseases and zoonotic Salmonella. hLife 2024; 2: 246–256.9.Status and challenges of global antimicrobial resistance control: A dialogue between Professors Yonghong Xiao and Takeshi Nishijima通信作者肖永红引用Xiao Y, Nishijima T. Status and challenges of global antimicrobial resistance control: A dialogue between Professors Yonghong Xiaoand Takeshi Nishijima. hLife 2024; 2: 47–49.
hLife Collection | Antimicrobial resistance
1. Understanding carbapenem-resistant hypervirulent Klebsiella pneumoniae: Key virulence factors and evolutionary convergencehLife | 破解耐药高毒力肺炎克雷伯菌毒力因子与进化机制揭秘通信作者肖永红本文系统阐述了耐碳青霉烯类高毒力肺炎克雷伯菌CR-hvKP的关键毒力因子及其进化整合机制。CR-hvKP的毒力相关基因分布于核心基因组染色体及辅助基因组如质粒、整合性接合元件 [ICEKp]中。这些遗传元件编码荚膜、脂多糖、铁载体、1/3型菌毛及基因毒素柯杆菌素colibactin等毒力因子赋予菌株高致病潜力。 CR-hvKP的形成源于持续的进化压力以及携带碳青霉烯酶基因或高毒力基因的质粒交换。目前认为耐药与高毒力表型在同一菌株中汇合的机制主要有三种1碳青霉烯耐药质粒水平转移至高毒力肺炎克雷伯菌2高毒力质粒转移至耐碳青霉烯肺炎克雷伯菌3菌株获得同时携带耐药及毒力基因的嵌合/杂合质粒。目前耐药与高毒力谱系在单一菌株中的融合现象日益增多对公共卫生构成严重威胁。CR-hvKP的广泛流行主要归因于其高度可变的基因组这种可塑性源于点突变、同源重组以及质粒、插入序列、原噬菌体等多种可移动遗传元件的频繁转移。引用Chen T, Ying L, Xiong L, et al. Understanding carbapenem-resistant hypervirulent Klebsiella pneumoniae: Key virulence factors and evolutionary convergence. hLife 2024; 2: 611–624.2. WHO revised bacterial priority pathogens list to encourage global actions to combat AMR通信作者肖永红The World Health Organization (WHO) has updated its Bacterial Priority Pathogens List (BPPL) for 2024 to combat antimicrobial resistance (AMR), focusing on One Health criteria such as treatability, mortality, and resistance trends. The list categorizes pathogens as critical, high, or medium priority, emphasizing the need for new antimicrobials. Notable inclusions are carbapenem-resistant Acinetobacter baumannii (CRAB), carbapenem-resistant Enterobacterales (CRE), and third-generation cephalosporin-resistant Enterobacterales (3GCRE). The AMR crisis, especially with multidrug-resistant gram-negative bacteria, persists, highlighting the urgency for global action, investment, and RD in diagnostics and therapeutics.引用Ma Y, Chen P, Mo Y, et al. WHO revised bacterial priority pathogens list to encourage global actions to combat AMR. hLife 2024; 2: 607–610.3. Personalized bacteriophage therapy for chronic biliary tract Pseudomonas aeruginosa infectionshLife | 胡必杰与乐率等研究团队合作发现对抗超级细菌的利器首例超级细菌胆道感染的噬菌体治疗案例通信作者朱同玉胡必杰乐率本研究首次证实了噬菌体疗法在胆道感染中的安全性和有效性为治疗提供了新的思路。尽管完全清除感染仍具挑战但噬菌体疗法在缓解症状和减少细菌载量方面效果显著。引用Li N, Li L, He B, et al. Personalized bacteriophage therapy for chronic biliary tract Pseudomonas aeruginosa infections. hLife 2025; 3: 275–283.4. RmpA drives metabolic reprogramming to modulate the phenotypic switch between hypermucoviscosity and biofilm formation in hypervirulent Klebsiella pneumoniaehLife | 冯婕研究团队揭示RmpA是操控高毒力肺炎克雷伯菌在高粘液性与生物膜间切换的代谢开关通信作者冯婕本文通过在高毒力肺炎克雷伯菌中构建针对rmpA基因的dCas9敲低菌株结合RNA-seq和ChIP-seq技术揭示了RmpA作为“代谢中枢调控器”的双重功能。引用Yao S, Huang J, Geng J, et al. RmpA drives metabolic reprogramming to modulate the phenotypic switch between hypermucoviscosity and biofilm formation in hypervirulent Klebsiella pneumoniae. hLife 2025; 3: 504–516.5. Carbapenem-resistant Klebsiella pneumoniae ST11 index from a single strain enhances rapid parallel evolution during persistent infectionhLife | 深圳三院曲久鑫研究团队揭示高毒高耐肺炎克雷伯菌ST11持续感染过程中的宿主内进化通信作者曲久鑫本文通过全基因组测序WGS分析了从一名持续感染患者体内时序性分离的一系列肺炎克雷伯菌菌株揭示并表征了肺炎克雷伯菌基因组、耐药表型和毒力在宿主内分化和适应过程并呼吁借鉴病毒学从准种理论的角度认识病原细菌持续性感染。引用Li L, Jiang Z, Wang X, et al. Carbapenem-resistant Klebsiella pneumoniae ST11 index from a single strain enhances rapid parallel evolution during persistent infection. hLife 2025; 3: 517–520.6.Heterogeneity and clinical genomics of blaKPC-2-producing, carbapenem-resistant Pseudomonas aeruginosahLife | 揭秘产blaKPC-2的碳青霉烯耐药铜绿假单胞菌的异质性与临床基因组学之谜通信作者黄曼、冯友军本文报道了KPC-2耐药质粒在碳青霉烯类耐药铜绿假单胞菌CRPA中的分布和遗传特征为研究我国临床ICU环境中CRPA传播机制提供了重要的基因组学视角。引用Huang M, Liu L, Li X, et al. Heterogeneity and clinical genomics of blaKPC-2-producing, carbapenem-resistant Pseudomonas aeruginosa. hLife 2024; 2: 314–319.7.Convergence of carbapenem resistance and hypervirulence in Klebsiella pneumoniae: An emerging public health threathLife | 高毒高耐肺炎克雷伯菌的全球播散及应对策略通信作者王明贵高耐高毒肺炎克雷伯菌的出现和广泛传播是潜在的公共卫生危机这类菌株正悄然改变临床感染格局。未来研究和防控策略可聚焦于基于CRISPR-Cas或毒素-抗毒素系统的去定植研究、针对毒力因子的靶向治疗研究、结合新型分子检测与监测网络的早期预警系统的建立等。只有通过加强基础研究、研发诊断手段、推动多学科协作才能有效遏制其蔓延守护临床安全与公共卫生防线。引用Jiang J, Zhang J, Sun Z, et al. Convergence of carbapenem resistance and hypervirulence in Klebsiella pneumoniae: An emerging public health threat. hLife 2025; 3: 501–503.8. Impact of COVID-19-related nonpharmaceutical interventions on diarrheal diseases and zoonotic SalmonellahLife | COVID-19相关非药物干预措施对急性腹泻和人畜共患沙门菌的影响通信作者乐敏引用Huang L, Zhou H, Chen J, et al. Impact of COVID-19-related nonpharmaceutical interventions on diarrheal diseases and zoonotic Salmonella. hLife 2024; 2: 246–256.9.Status and challenges of global antimicrobial resistance control: A dialogue between Professors Yonghong Xiao and Takeshi Nishijima通信作者肖永红引用Xiao Y, Nishijima T. Status and challenges of global antimicrobial resistance control: A dialogue between Professors Yonghong Xiaoand Takeshi Nishijima. hLife 2024; 2: 47–49.
